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, Linzhou Huang Department of Plant and Microbial Biology, North Carolina State University , Raleigh, NC 27695 , USA Author for correspondence: mrojasp@ncsu.edu (M.R.-P.), lzhuang@zafu.edu.cn (L.H.); Present address: College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China. Search for other works by this author on: Oxford Academic Marcela Rojas-Pierce Department of Plant and Microbial Biology, North Carolina State University , Raleigh, NC 27695 , USA Author for correspondence: mrojasp@ncsu.edu (M.R.-P.), lzhuang@zafu.edu.cn (L.H.); Present address: College of Advanced Agricultural Sciences, Zhejiang A&F University, Hangzhou, Zhejiang 311300, China. Search for other works by this author on: Oxford Academic
The Plant Cell, Volume 36, Issue 9, September 2024, Pages 3145–3161, https://doi.org/10.1093/plcell/koae072
Published:
06 March 2024
Article history
Received:
18 August 2023
Accepted:
26 February 2024
Published:
06 March 2024
Corrected and typeset:
20 March 2024
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Linzhou Huang, Marcela Rojas-Pierce, Rapid depletion of target proteins in plants by an inducible protein degradation system, The Plant Cell, Volume 36, Issue 9, September 2024, Pages 3145–3161, https://doi.org/10.1093/plcell/koae072
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Abstract
Inducible protein knockdowns are excellent tools to test the function of essential proteins in short time scales and to capture the role of proteins in dynamic events. Current approaches destroy or sequester proteins by exploiting plant biological mechanisms such as the activity of photoreceptors for optogenetics or auxin-mediated ubiquitination in auxin degrons. It follows that these are not applicable for plants as light and auxin are strong signals for plant cells. We describe here an inducible protein degradation system in plants named E3-DART for E3-targeted Degradation of Plant Proteins. The E3-DART system is based on the specific and well-characterized interaction between the Salmonella-secreted protein H1 (SspH1) and its human target protein kinase N1 (PKN1). This system harnesses the E3 catalytic activity of SspH1 and the SspH1-binding activity of the hom*ology region 1b (HR1b) domain from PKN1. Using Nicotiana benthamiana and Arabidopsis (Arabidopsis thaliana), we show that a chimeric protein containing the leucine-rich repeat and novel E3 ligase domains of SspH1 efficiently targets protein fusions of varying sizes containing HR1b for degradation. Target protein degradation was induced by transcriptional control of the chimeric E3 ligase using a glucocorticoid transactivation system, and target protein depletion was detected as early as 3 h after induction. This system could be used to study the loss of any plant protein with high-temporal resolution and may become an important tool in plant cell biology.
© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.
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